Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Cromosomas Politénicos by Diana Monroy on Prezi

Caliente para dispersar a la Triton X y su uso dentro de una hora. Unable to load video. Agradecemos a la Sra. Fill out the form below to receive a free trial or learn more about access:.

Get cutting-edge science videos from J o VE politenios straight to your inbox every month. Your institution must subscribe to JoVE’s Biology section to access this content.

For other languages click here. This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect?

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes. An unexpected error occurred. Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromodomas.


Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos. You are right about the Triton.

Las modificaciones de este procedimiento se describen en detalle en Johansen et al. If you leave the glands in too long they will fall apart.

Please sign in or create an account. You will only be able to see the first 20 seconds. That is the volume I prepare each time, usually enough for two or three prep. Click here for the english version. If the problem continues, please let us know and we’ll try to cromosomae. Repita los pasos 4. The trick is to leave them in just long enough. Please check your Internet connection and reload this page. Please recommend JoVE to your librarian.

Deficiencia intelectual – catarata – cifosis

My thought is that they may be getting flushed out during my transfers. Skip to content Biology. A subscription to J o VE is required to view this article. If you are seeing few salivary gland chromosome sets you may be losing them when you squash.


The dissection forceps we use were mis-identified: Lugar pesos g en el cubreobjetos de las diapositivas. If you have any question you can send me another e-mail. Do crompsomas have any advice? If that doesn’t help, please let us know. Is it the PFA? We recommend downloading the newest version of Flash here, but we support all versions 10 and above. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present.

You must be signed in to post a comment. Warm to disperse the Triton X Fill out the form below to receive a free trial or learn more about access: A crromosomas that is too rapid or hard may also cause the chromosomes to float away.