ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of.
|Published (Last):||22 April 2017|
|PDF File Size:||9.37 Mb|
|ePub File Size:||11.73 Mb|
|Price:||Free* [*Free Regsitration Required]|
Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing anaerobic bacteria involved in infectious diseases. Previous versions of documents Veterinary Susceptibility Testing International Organization for Standards, Geneva, Switzerland. Clinical and Laboratory Standards Institute. Search all products by. However, preparation of ITC trays for antimicrobial susceptibility testing AST microdilution tests is associated with a significant lack of reproducibility, particularly after freezing and shipping, independent of which dilution method was followed.
ECDC documents of relevance to antimicrobial susceptibility testing. Although clinical interpretation of the MIC value is beyond the scope of this part of ISOmodifications of the basic method are required for certain antimicrobial agent – bacteria combinations to facilitate clinical interpretation.
Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices Evaluation of performance of antimicrobial susceptibility test devices BS EN Find Similar Items This product falls into the following categories. Dilution procedures are used to determine the minimum inhibitory concentrations MICs of antimicrobial agents and are the reference method for antimicrobial susceptibility testing.
In dilution tests, microorganisms are tested for their ability to produce visible growth on a series of agar plates agar dilution or in broth broth dilution containing serial dilutions of the antimicrobial agent.
Reference method for broth dilution antifungal susceptibility testing of filamentous fungi, 2nd ed. All these methods are based on those described by Ericsson and Sherris.
HPLC is a sensitive and reproducible method for determining levels of azole components in a great variety of matrices, as has been confirmed in previous reports 12510 – 121519 – 21 I agree More information.
Where clinical data is lacking! Itraconazole formulation studies of the melt-extrusion process with mixture design. Rapid AST in bloodcultures Support Center Support Center. Referenzmethoden zur 2077-1 der In-vitro-Aktivitaet von antimikrobiellen Substanzen gengen Bakterien, die Infektionskrankheiten verursachen. Methode de reference pour la determination de la sensibilite in vitro aux agents microbiens des bacteries aerobies a croissance rapide impliquees dans les maladies infectieuses.
Breakpoints for short incubation Expert rules and intrinsic resistance Resistance mechanisms Guidance documents Consultations – New!
EUCAST: External documents
BS EN ISO 20776-1:2006
The method described in this part of ISO is intended for the testing of pure cultures of aerobic bacteria that are easily grown by overnight incubation on agar and grow well in Mueller-Hinton broth, which may be supplemented. In order to determine and compare the accuracy of each method described for microdilution, two consecutive HPLC determinations of the azole concentration were calculated from different wells of trays prepared according ISO recommendations, serial dilutions for 220776-1 drugs, and serial dilutions for hydrophilic compounds.
These documents are available for purchase from ISO or national standards organisations. Susceptibility testing of pathogenic fungi with itraconazole: Acknowledgments We have no conflict of interest to declare. Abstract A multicenter study was conducted to 220776-1 the accuracy of the ISO standard and the serial 2-fold ixo procedures for antifungal susceptibility testing.
The presence and concentration of FLC or ITC in ios well was determined by comparing the chromatographic signal peak area, retention time, and a UV spectral pattern of between and nm from the sample and from standard solutions. Author information Article notes Copyright and License information Disclaimer. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility testing devices.
The greatest discrepancy was observed for the lowest concentrations and for the dilutions prepared outside Spain and shipped for concentration determinations. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices.
MIC methods are used in resistance surveillance, comparative testing of new agents, to establish the susceptibility of organisms that give equivocal results in routine tests, for tests on organisms where routine tests may be unreliable and when a quantitative result is required for clinical management. This article has been cited by other articles in PMC.
Susceptibility testing of moulds You are advised to check costs as they may differ substantially between suppliers. Careful control and standardisation is required for intra- and inter-laboratory reproducibility, as results may be significantly influenced by the method used.
It is generally accepted that broth MIC tests are reproducible to within one doubling dilution of the real end point i.
Received Jan 20; Accepted Feb